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SRX26772312: Uni18SS3
1 ILLUMINA (Illumina HiSeq 3000) run: 4,190 spots, 1.9M bases, 641,670b downloads

Design: Three commonly used primer pairs were utilized in this study, AcMDB07-F and AcMDB07-R for the fish 12S rRNA gene, Uni18S and Uni18SR for the eu-karyotic hypervariable V4 region of the 18S rRNA gene, and TAReuk454FWD1 and TAReukREV3 for the eukaryotic hypervariable V4 region of the 18S rRNA gene.
Submitted by: Guangdong Meilikang Bio-Science Ltd.
Study: Detection of feed biological raw materials and potential eu-karyotic pathogens in feed ingredients based on high-throughput sequencing
show Abstracthide Abstract
To assess the effectiveness of high-throughput sequencing technology in detecting both feed raw materials and potential eukaryotic pathogens, this study evaluated the eukaryotic composition of raw materials using three primer pairs of eukaryotic primers. Our results showed significant variations in species annotation among the sequences obtained from different primers. Specifi-cally, the 18S rRNA gene primer pairs (Uni18S and Uni18SR, and TAReuk454FWD1 and TAReukREV3) were able to detect a wide range of species and particularly effective for identifying fungi, whereas the 12S rRNA gene primer pair (AcMDB07-F and AcMDB07-R) provided more detailed identifications of multicellular ingredient sources.
Sample: Uni18SS3
SAMN44828737 • SRS23259378 • All experiments • All runs
Organism: metagenome
Library:
Name: Uni18SS3
Instrument: Illumina HiSeq 3000
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 4,190 spots, 1.9M bases, 641,670b
Run# of Spots# of BasesSizePublished
SRR314032044,1901.9M641,670b2024-11-19

ID:
36180428

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